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ERIC Number: EJ920086
Record Type: Journal
Publication Date: 2010-Sep
Pages: 3
Abstractor: As Provided
Reference Count: 25
ISBN: N/A
ISSN: ISSN-0021-9584
Biocatalysis with Sol-Gel Encapsulated Acid Phosphatase
Kulkarni, Suhasini; Tran, Vu; Ho, Maggie K.-M.; Phan, Chieu; Chin, Elizabeth; Wemmer, Zeke; Sommerhalter, Monika
Journal of Chemical Education, v87 n9 p958-960 Sep 2010
This experiment was performed in an upper-level undergraduate biochemistry laboratory course. Students learned how to immobilize an enzyme in a sol-gel matrix and how to perform and evaluate enzyme-activity measurements. The enzyme acid phosphatase (APase) from wheat germ was encapsulated in sol-gel beads that were prepared from the precursor molecule tetramethoxysilane. The enzymatic activities of free and immobilized APase were measured with a colorimetric assay that monitors the formation of "p"-nitrophenolate from "p"-nitrophenylphosphate. Students critically assessed the advantages and disadvantages of sol-gel based enzyme immobilization by investigating the catalytic performance, the enzyme retention, and the reuse capability of the APase sol-gel beads. This experiment exposed students to thoughts and techniques that are important for the biotechnological application of enzymes. (Contains 2 notes, 1 figure, and 2 schemes.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A