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ERIC Number: EJ920085
Record Type: Journal
Publication Date: 2010-Sep
Pages: 2
Abstractor: As Provided
Reference Count: 2
ISBN: N/A
ISSN: ISSN-0021-9584
An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory
Olsen, Robert J.; Olsen, Julie A.; Giles, Greta A.
Journal of Chemical Education, v87 n9 p956-957 Sep 2010
An experiment using [superscript 1]H NMR spectroscopy to observe the kinetics of the acylase 1-catalyzed hydrolysis of "N"-acetyl-DL-methionine has been developed for the organic laboratory. The L-enantiomer of the reactant is hydrolyzed completely in less than 2 h, and [superscript 1]H NMR spectroscopic data from a single sample can be worked up to generate Michaelis-Menten and Lineweaver-Burke kinetic plots. The experiment introduces organic chemistry students to the use of a [superscript 1]H NMR spectrometer, serves as an introduction to enzyme kinetics, and illustrates the stereospecificity of enzyme catalysis. (Contains 4 figures and 3 notes.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A