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ERIC Number: EJ918443
Record Type: Journal
Publication Date: 2010-Jul
Pages: 4
Abstractor: As Provided
Reference Count: 20
ISSN: ISSN-0021-9584
Complementary Spectroscopic Assays for Investigating Protein-Ligand Binding Activity: A Project for the Advanced Chemistry Laboratory
Mascotti, David P.; Waner, Mark J.
Journal of Chemical Education, v87 n7 p735-738 Jul 2010
A protein-ligand binding, guided-inquiry laboratory project with potential application across the advanced undergraduate curriculum is described. At the heart of the project are fluorescence and spectrophotometric assays utilizing biotin-4-fluorescein and streptavidin. The use of the same stock solutions for an assay that may be examined by two distinct spectroscopic techniques offers an opportunity to discuss not only protein-ligand binding but also to compare instrumental techniques and to discuss the underlying physical principles that are the origins of the assays. In addition to critically evaluating analytical techniques, students are pushed to develop quantitative pipetting, estimation, and experimental-design skills to collect and analyze their data. (Contains 3 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A