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ERIC Number: EJ913313
Record Type: Journal
Publication Date: 2010-Mar
Pages: 3
Abstractor: As Provided
Reference Count: 12
ISBN: N/A
ISSN: ISSN-0021-9584
Expression and Purification of Sperm Whale Myoglobin
Miller, Stephen; Indivero, Virginia; Burkhard, Caroline
Journal of Chemical Education, v87 n3 p303-305 Mar 2010
We present a multiweek laboratory exercise that exposes students to the fundamental techniques of bacterial expression and protein purification through the preparation of sperm whale myoglobin. Myoglobin, a robust oxygen-binding protein, contains a single heme that gives the protein a reddish color, making it an ideal subject for the teaching laboratory as students can follow the progress of the protein visually in real time. The purification uses both ion-exchange and size-exclusion chromatography, thus, introducing students to two of the most common separation methods. Students evaluate the quality of the purification via sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In most cases, this procedure produces sufficient quantities of pure protein for further laboratory exercises studying the properties of myoglobin. (Contains 4 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A