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ERIC Number: EJ913227
Record Type: Journal
Publication Date: 2010-Feb
Pages: 4
Abstractor: As Provided
Reference Count: 25
ISBN: N/A
ISSN: ISSN-0021-9584
Identification of Fatty Acids, Phospholipids, and Their Oxidation Products Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Electrospray Ionization Mass Spectrometry
Harmon, Christopher W.; Mang, Stephen A.; Greaves, John; Finlayson-Pitts, Barbara J.
Journal of Chemical Education, v87 n2 p186-189 Feb 2010
Electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) have found increasing application in the analysis of biological samples. Using these techniques to solve problems in analytical chemistry should be an essential component of the training of undergraduate chemists. We present an experiment that teaches students how to prepare and analyze samples both qualitatively and quantitatively using modern mass spectrometric techniques. Students use ESI-MS and MALDI-MS to analyze phospholipids and mixtures of fatty acids, including those from soybean extracts. Changes in the mixtures upon ozonolysis are also investigated. This laboratory is suitable for upper-level university students enrolled in analytical chemistry or instrumental analysis classes. This experiment combines analytical chemistry with organic chemistry and biochemistry, a situation that students will encounter in their future careers as chemists. (Contains 5 figures and 1 table.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A