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ERIC Number: EJ895539
Record Type: Journal
Publication Date: 2010
Pages: 5
Abstractor: As Provided
ISSN: ISSN-1470-8175
RFLP Analysis and Allelic Discrimination with Real-Time PCR Using the Human Lactase Persistence Trait: A Pair of Molecular Genetic Investigations
Weinlander, Kenneth M.; Hall, David J.; De Stasio, Elizabeth A.
Biochemistry and Molecular Biology Education, v38 n3 p167-171 May-Jun 2010
We describe here two open-ended laboratory investigations for an undergraduate laboratory course that uses students' DNA as templates for quantitative real-time PCR and for traditional PCR followed by RFLP analysis. Students are captivated by the immediacy of the application and the relevance of the genotypes and traits, lactase persistence or nonpersistence, under study. Concepts ranging from nucleotide polymorphisms to natural selection can be demonstrated and reinforced by these laboratories. Students have a high degree of success with the laboratories and both objective and self-assessments of student learning indicate that concepts and techniques are better understood following implementation of these laboratory investigations. (Contains 2 figures and 1 table.)
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Wisconsin