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ERIC Number: EJ877840
Record Type: Journal
Publication Date: 2010
Pages: 6
Abstractor: As Provided
Reference Count: 19
ISSN: ISSN-1470-8175
Using Paraquat to Generate Anion Free Radicals and Hydrogen Peroxide in "In Vitro": Antioxidant Effect of Vitamin E--A Procedure to Teach Theoretical and Experimental Principles of Reactive Oxygen Species Biochemistry
Jimenez-Del-Rio, Marlene; Suarez-Cedeno, Gerson; Velez-Pardo, Carlos
Biochemistry and Molecular Biology Education, v38 n2 p104-109 Mar-Apr 2010
The theoretical basis of reactive oxygen species and their impact on health issues are relatively easy to understand by biomedical students. The detection of reactive oxygen species requires expensive equipment, the procedures are time consuming and costly, and the results are hard to interpret. Moreover, cause-and-effect relationships in the living system are not so evident. In this report, we adapted a two-step procedure to detect anion superoxide radicals and hydrogen peroxide generation in lymphocytes exposed to paraquat by using nitroblue tetrazolium salt and dihydrorhodamine, respectively. Also, a two-step assay was performed to evaluate lymphocyte viability and nuclei morphologic changes on paraquat exposure for 1 and 24 hours incubation time by using trypan blue exclusion assay and acridine orange/ethidium bromide staining technique, respectively. Vitamin E was used as antioxidant to inhibit the deleterious effects of paraquat on cells. Students learned how to (i) design and perform experiments in the laboratory, (ii) read critical scientific literature, and (iii) discuss and contrast relevant information about reactive oxygen species as causative agents of cell death phenomenon. (Contains 3 figures and 1 table.)
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Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Colombia