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ERIC Number: EJ867627
Record Type: Journal
Publication Date: 2009
Pages: 5
Abstractor: As Provided
Reference Count: 4
ISSN: ISSN-1470-8175
Using "Pseudomonas Putida xylE" Gene to Teach Molecular Cloning Techniques for Undergraduates
Dong, Xu; Xin, Yi; Ye, Li; Ma, Yufang
Biochemistry and Molecular Biology Education, v37 n6 p339-343 Nov-Dec 2009
We have developed and implemented a serial experiment in molecular cloning laboratory course for undergraduate students majored in biotechnology. "Pseudomonas putida xylE" gene, encoding catechol 2, 3-dioxygenase, was manipulated to learn molecular biology techniques. The integration of cloning, expression, and enzyme assay gave students a chance to mimic real research and product development by connecting separated techniques. This serial experiment helped students set up intact idea of molecular biology and to understand the relation of each individual operation. (Contains 6 figures and 2 tables.)
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Students
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: China