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ERIC Number: EJ858366
Record Type: Journal
Publication Date: 2009
Pages: 6
Abstractor: As Provided
Reference Count: 16
ISSN: ISSN-1470-8175
[beta]-Lactamases in the Biochemistry and Molecular Biology Laboratory
Amador, Paula; Prudencio, Cristina; Vieira, Monica; Ferraz, Ricardo; Fonte, Rosalia; Silva, Nuno; Coelho, Pedro; Fernandes, Ruben
Biochemistry and Molecular Biology Education, v37 n5 p301-306 Sep-Oct 2009
[beta]-lactamases are hydrolytic enzymes that inactivate the [beta]-lactam ring of antibiotics such as penicillins and cephalosporins. The major diversity of studies carried out until now have mainly focused on the characterization of [beta]-lactamases recovered among clinical isolates of Gram-positive staphylococci and Gram-negative enterobacteria, amongst others. However, only some studies refer to the detection and development of [beta]-lactamases carriers in healthy humans, sick animals, or even in strains isolated from environmental stocks such as food, water, or soils. Considering this, we proposed a 10-week laboratory programme for the Biochemistry and Molecular Biology laboratory for majors in the health, environmental, and agronomical sciences. During those weeks, students would be dealing with some basic techniques such as DNA extraction, bacterial transformation, polymerase chain reaction (PCR), gel electrophoresis, and the use of several bioinformatics tools. These laboratory exercises would be conducted as a mini research project in which all the classes would be connected with the previous ones. This "curriculum" was compared in an experiment involving two groups of students from two different majors. The new "curriculum", with classes linked together as a mini research project, was taught to a major in Pharmacy and an old "curriculum" was taught to students from environmental health. The results showed that students who were enrolled in the new "curriculum" obtained better results in the final exam than the students who were enrolled in the former "curriculum". Likewise, these students were found to be more enthusiastic during the laboratory classes than those from the former "curriculum". (Contains 2 figures and 2 tables.)
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Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Portugal