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ERIC Number: EJ855069
Record Type: Journal
Publication Date: 2009
Pages: 3
Abstractor: As Provided
Reference Count: 5
ISBN: N/A
ISSN: ISSN-1084-6522
Evidence of Possible Evolutionary Divergence in Plant Genera Based on Antioxidant Properties
Asai, Elizabeth; Cao, Sharon
NCSSSMST Journal, v14 n2 p10-12 Spr 2009
The purpose of this investigation was to determine if three Western species of the Panax, Lycium, and Astragalus genera had antibacterial and/or antioxidant properties, and how their properties compared to Eastern herbs in the same genera. The group hypothesized that when compared, the corresponding herbs would have identical antibacterial and antioxidant properties. An antibacterial disc test was used to determine each herb's ability to inhibit the growth of "Escherichia coli and Micrococcus luteus." Herb and bacteria pairs which were not antibacterial were tested in a UV exposure test, a method used to qualify antioxidant properties. Mixtures of bacterial broth and herbal teas were exposed to UV light, and plated onto agar. Survival of the bacteria was observed. In addition, to determine if color of the herb impacted the protection of the bacteria, Nigrosin, a black stain, was tested under the same UV exposure procedure. In a DPPH test, the color change of the DPPH was used to calculate radical scavenging activity as a measure of antioxidant strength. The corresponding herbs' data were then compared. For the antibacterial test, only the known antibiotic control, Streptomycin, yielded a zone of inhibition. All of the herb and bacteria pairs showed antioxidant properties after UV exposure. The DPPH test found that the herb from the "Lycium" genus had the most radical scavenging activity, followed by the "Astragalus" and "Panax" genera. When compared to the data from Eastern species of these herbs, all three of the Western herbs were found to have greater antioxidant properties, especially in the Lycium genus. In the wolfberry trials, the Eastern wolfberry had a radical scavenging activity of 65.0% and the Western herb had 93.1% scavenging activity (Har & Seetoh, 2008). (Contains 1 table.)
National Consortium for Specialized Secondary Schools of Mathematics, Science and Technology. 3020 Wards Ferry Road, Lynchburg, VA 24502. Tel: 434-582-1104; Fax: 434-239-4140; e-mail: office@ncsssmst.org; Web site: http://www.ncsssmst.org
Publication Type: Journal Articles; Reports - Evaluative
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Singapore; Virginia