NotesFAQContact Us
Collection
Advanced
Search Tips
Peer reviewed Peer reviewed
Direct linkDirect link
ERIC Number: EJ850689
Record Type: Journal
Publication Date: 2009
Pages: 6
Abstractor: As Provided
Reference Count: 9
ISBN: N/A
ISSN: ISSN-1470-8175
A Simple Test Tube-Based ELISA Experiment for the High-School Classroom
Brokaw, Ann; Cobb, Brian A.
Biochemistry and Molecular Biology Education, v37 n4 p243-248 Jul-Aug 2009
Immunology is gaining prominence both in the media as well as on the Advanced Placement (AP) exam in Biology. One of the challenges of teaching modern biological topics such as immunology and biochemistry in the high-school setting is the increased reliance on expensive technology in the research world. To begin to bridge this widening gap, we devised an experiment using a novel macroscale enzyme-linked immunosorbent assay that is suitable for AP-level high-school biology classrooms as well as entry-level collegiate laboratories. This novel method does not require a plate reader for data analysis, but instead relies on more common and inexpensive equipment such as a clinical test tube centrifuge and a simple test tube spectrophotometer. The experimental plan focuses on students measuring antibody concentrations in "unknown" samples and includes the collection and analysis of a standard curve using reagents prepared by the instructor. Students will be introduced to enzyme action, quantitative laboratory technique, antibodies, and the immune system, with the overall goal being to explore and highlight the inherent connections within the fields of biochemistry and immunology. (Contains 2 figures, 3 schemes and 1 table.)
John Wiley & Sons, Inc. Subscription Department, 111 River Street, Hoboken, NJ 07030-5774. Tel: 800-825-7550; Tel: 201-748-6645; Fax: 201-748-6021; e-mail: subinfo@wiley.com; Web site: https://secure.interscience.wiley.com/cgi-bin/jhome/112782101
Publication Type: Journal Articles; Reports - Descriptive
Education Level: High Schools
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A