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ERIC Number: EJ848550
Record Type: Journal
Publication Date: 2009-Jun
Pages: 10
Abstractor: As Provided
Reference Count: 0
ISSN: ISSN-1092-4388
The Cystic Fibrosis Transmembrane Conductance Regulator and Chloride-Dependent Ion Fluxes of Ovine Vocal Fold Epithelium
Leydon, Ciara; Fisher, Kimberly V.; Lodewyck-Falciglia, Danielle
Journal of Speech, Language, and Hearing Research, v52 n3 p745-754 Jun 2009
Purpose: Ion-driven transepithelial water fluxes participate in maintaining superficial vocal fold hydration, which is necessary for normal voice production. The authors hypothesized that Cl[superscript -] channels are present in vocal fold epithelial cells and that transepithelial Cl[superscript -] fluxes can be manipulated pharmacologically. Method: Immunohistochemical assays were used to identify cystic fibrosis transmembrane regulator Cl[superscript -] channels in ovine vocal fold mucosae (n = 2). Electrophysiological responses of vocal fold mucosae (n = 80) to Cl[superscript -] channel inhibitors and secretagogues were evaluated in an ovine model using a randomized controlled experimental design. Results: Cystic fibrosis transmembrane regulator channels were localized to the plasma membranes of epithelial cells. The Cl[superscript -] transport inhibitor, diphenylamine-2-carboxylate, elicited a 30% decrease in mean short-circuit current (I[subscript sc]; n = 10). The secretagogue, isobutylmethylxanthine, yielded a 31.7% increase in mean I[subscript sc] (n = 10). Another secretagogue, uridine triphosphate, elicited a 48.8% immediate and 17.3% sustained increase in mean I[subscript sc] (n = 10). No sustained increases occurred following application of secretagogues to mucosae bathed in a low Cl[superscript -] environment (n = 10), suggesting that responses were Cl[superscript -] dependent. Conclusions: The authors provide structural and functional evidence for the presence of a transepithelial pathway for Cl[superscript -] fluxes. Pharmacological manipulation of this pathway may offer a mechanism for maintaining superficial vocal fold hydration.
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Publication Type: Journal Articles; Reports - Research
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A