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ERIC Number: EJ846058
Record Type: Journal
Publication Date: 2009
Pages: 7
Abstractor: As Provided
Reference Count: 16
ISSN: ISSN-1931-7913
A Study of Rubisco through Western Blotting and Tissue Printing Techniques
Ma, Zhong; Cooper, Cynthia; Kim, Hyun-Joo; Janick-Buckner, Diane
CBE - Life Sciences Education, v8 n2 p140-146 Sum 2009
We describe a laboratory exercise developed for a cell biology course for second-year undergraduate biology majors. It was designed to introduce undergraduates to the basic molecular biology techniques of Western blotting and immunodetection coupled with the technique of tissue printing in detecting the presence, relative abundance, and distribution of ribulose-1,5-bisphosphate carboxylase in various plant materials. Pre- and postlab surveys indicated significant postlab gains in student understanding of all three lab techniques and relevant lecture topics. Additional postlab survey questions on student perception of the lab modules suggested that the laboratory exercises successfully met a series of pedagogical goals set by the instructors. The combination of these techniques provided a basis for quantitative and qualitative (visual) analysis of a biologically important enzyme and can be applied or modified readily to study other proteins and biological molecules in lab exercises for an introductory cell biology course or molecular biology course. (Contains 4 figures and 2 tables.)
American Society for Cell Biology. 8120 Woodmont Avenue Suite 750, Bethesda, MD 20814-2762. Tel: 301-347-9300; Fax: 301-347-9310; E-mail:; Website:
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A