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ERIC Number: EJ832486
Record Type: Journal
Publication Date: 2009-Mar
Pages: 3
Abstractor: As Provided
Reference Count: 7
ISBN: N/A
ISSN: ISSN-0021-9584
The Isolation of Invertase from Baker's Yeast: A Four-Part Exercise in Protein Purification and Characterization
Timerman, Anthony P.; Fenrick, Angela M.; Zamis, Thomas M.
Journal of Chemical Education, v86 n3 p379-381 Mar 2009
A sequence of exercises for the isolation and characterization of invertase (E.C. 3.1.2.26) from baker's yeast obtained from a local grocery store is outlined. Because the enzyme is colorless, the use of colored markers and the sequence of purification steps are designed to "visualize" the process by which a colorless protein is selectively detected and isolated from a mixture of many different proteins. Students then compare their product from each stage of the purification against a commercial standard of purified invertase using both qualitative (SDS-PAGE) and quantitative (specific activity) techniques. The equipment and materials required for this 4-week exercise are either already available in most undergraduate departments or are not cost prohibitive. Our experience suggests that this project is well suited to undergraduate institutions limited to a one-semester course in biochemistry that caters to a large and diverse student enrollment. (Contains 2 tables and 2 figures.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.org
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A