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ERIC Number: EJ829539
Record Type: Journal
Publication Date: 2008-Sep
Pages: 4
Abstractor: As Provided
Reference Count: 4
ISSN: ISSN-0021-9584
Optimizing Chromatographic Separation: An Experiment Using an HPLC Simulator
Shalliker, R. A.; Kayillo, S.; Dennis, G. R.
Journal of Chemical Education, v85 n9 p1265-1268 Sep 2008
Optimization of a chromatographic separation within the time constraints of a laboratory session is practically impossible. However, by employing a HPLC simulator, experiments can be designed that allow students to develop an appreciation of the complexities involved in optimization procedures. In the present exercise, a HPLC simulator from "JCE Software" is used to optimize the separation of a nine-component mixture. The relationship between solvent composition and retention factor is measured to determine the solvent composition for maximized separation. Use of Excel spreadsheets assists in the analysis of the data required for the optimization process. As a result, this exercise demonstrates the basics of the iterative process for optimization of separations and application of spreadsheets to solve relatively complex and tedious repetitive problems. (Contains 7 figures.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A