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ERIC Number: EJ829532
Record Type: Journal
Publication Date: 2008-Sep
Pages: 3
Abstractor: As Provided
Reference Count: 10
ISBN: N/A
ISSN: ISSN-0021-9584
A Computer-Interfaced Drop Counter as an Inexpensive Fraction Collector for Column Chromatography
Nash, Barbara T.
Journal of Chemical Education, v85 n9 p1260-1262 Sep 2008
A computer-interfaced drop counter is described that serves as an inexpensive alternative to a fraction collector for column chromatography experiments. Undergraduate biochemistry laboratories frequently do not have the budget to purchase fraction collectors. Protocols that call for the manual measurement of fraction volumes as well as the manual application of buffer to the column do not provide students with the skills required to function in a professional setting. In this experiment, students use a column outfitted with Luer fittings to set up an automatic buffer-feeding system and also monitor fraction collection using a drop counter. Students have the opportunity to learn troubleshooting techniques for both methods. To illustrate this system, a sample of typical student data from an experiment in which the molecular weight of myoglobin is determined by gel filtration chromatography is shown. The system described can be used for any column chromatography experiment, including preparative gel filtration chromatography, ion exchange chromatography, and affinity chromatography. (Contains 3 figures and 2 notes.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.org
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A