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ERIC Number: EJ827436
Record Type: Journal
Publication Date: 2008-Jul
Pages: 3
Abstractor: As Provided
Reference Count: 25
ISSN: ISSN-0021-9584
Separation of the Carotenoid Bixin from Annatto Seeds Using Thin-Layer and Column Chromatography
McCullagh, James V.; Ramos, Nicholas
Journal of Chemical Education, v85 n7 p948-950 Jul 2008
In this experiment the carotenoid bixin is isolated from annatto ("Bixa orellana") seeds using column chromatography. The experiment has several key advantages over previous pigment separation experiments. First, unlike other experiments significant quantities of the carotenoid (typically 20 to 25 mg) can be isolated from small quantities of plant material. Second, in this experiment students learn how to choose the appropriate solvent for a chromatographic separation by analyzing several potential solvents using thin-layer chromatography. After isolation, the purified bixin is analyzed by UV-vis and IR spectroscopy. (Contains 2 figures and 1 note.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A