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ERIC Number: EJ827434
Record Type: Journal
Publication Date: 2008-Jul
Pages: 2
Abstractor: As Provided
Reference Count: 6
ISSN: ISSN-0021-9584
The Preparation and Enzymatic Hydrolysis of a Library of Esters
Sanford, Elizabeth M.; Smith, Traci L.
Journal of Chemical Education, v85 n7 p944-945 Jul 2008
An investigative case study involving the preparation of a library of esters using Fischer esterification and alcoholysis of acid chlorides and their subsequent enzymatic hydrolysis by pig liver esterase and orange peel esterase is described. Students work collaboratively to prepare and characterize the library of esters and complete and evaluate the feasibility of the enzymatic hydrolysis for the goals of the case. Students make extensive use of [superscript 1]H NMR to characterize products and to determine the product or products of the enzymatic reaction. The interdisciplinary nature of this experiment in the context of a case study allows students to make connections to biology; see how chemical and biological approaches to a problem differ and complement each other; gain experience in a sequential three-step synthesis and the characterization of mixtures; and develop group skills and reflective judgment. (Contains 1 schema.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A