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ERIC Number: EJ826732
Record Type: Journal
Publication Date: 2008-May
Pages: 7
Abstractor: As Provided
Reference Count: 23
ISBN: N/A
ISSN: ISSN-0021-9584
A-DNA and B-DNA: Comparing Their Historical X-Ray Fiber Diffraction Images
Lucas, Amand A.
Journal of Chemical Education, v85 n5 p737-743 May 2008
A-DNA and B-DNA are two secondary molecular conformations (among other allomorphs) that double-stranded DNA drawn into a fiber can assume, depending on the relative water content and other chemical parameters of the fiber. They were the first two forms to be observed by X-ray fiber diffraction in the early 1950s, respectively by Wilkins and Gosling and by Franklin and Gosling. Their corresponding historical diffraction diagrams played an equally crucial role in the discovery of the primary double-helical structure of the DNA molecule by Watson and Crick in 1953. This paper provides a comparative explanation of the structural content of the two diagrams treated on the same footing. The analysis of the diagrams is supported by the optical transform method with which both A-DNA and B-DNA X-ray images can be simulated optically. The simulations use a simple laser pointer and a dozen optical diffraction gratings, all held on a single diffraction slide. The gratings have been specially designed to pinpoint just which of the structural elements of the molecule is responsible for each of the revealing features of the fiber diffraction images. (Contains 9 figures and 2 notes.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.org
Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A