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ERIC Number: EJ826721
Record Type: Journal
Publication Date: 2008-May
Pages: 3
Abstractor: As Provided
Reference Count: 15
ISSN: ISSN-0021-9584
Size Exclusion Chromatography: An Experiment for High School and Community College Chemistry and Biotechnology Laboratory Programs
Brunauer, Linda S.; Davis, Kathryn K.
Journal of Chemical Education, v85 n5 p683-685 May 2008
A simple multiday laboratory exercise suitable for use in a high school or community college chemistry course or a biotechnology advanced placement biology course is described. In this experiment students gain experience in the use of column chromatography as a tool for the separation and characterization of biomolecules, thus expanding their exposure to chromatographic procedures beyond those more commonly employed in the high school environment (e.g., paper or thin-layer chromatography). Students chromatographically separate a standard mixture of three highly colored compounds of varying molar mass (vitamin B[subscript 12], bovine hemoglobin, and blue dextran) on a short Sephacryl S-300 size exclusion column, determining their relative elution volumes. Students later apply a sample of the enzyme wheat germ acid phosphatase to the same column, collect eluant fractions and determine the elution position of the enzyme by use of a rapid and simple enzyme assay carried out in a 96 well plate. The relative molar mass of the enzyme is estimated by comparison of its elution volume to the elution profile of the molecules in the standard mixture. (Contains 2 figures.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: High Schools; Two Year Colleges
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A