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ERIC Number: EJ826718
Record Type: Journal
Publication Date: 2008-May
Pages: 2
Abstractor: As Provided
Reference Count: 5
ISBN: N/A
ISSN: ISSN-0021-9584
Metal-Catalyzed Cleavage of tRNA[superscript Phe]
Kirk, Sarah R.; Silverstein, Todd P.; McFarlane Holman, Karen L.
Journal of Chemical Education, v85 n5 p676-677 May 2008
This laboratory project is one component of a semester-long advanced biochemistry laboratory course that uses several complementary techniques to study tRNA[superscript Phe] conformational changes induced by ligand binding. In this article we describe a set of experiments in which students assay metal-catalyzed hydrolysis of tRNA[superscript Phe] using gel electrophoresis. tRNA[superscript Phe] cleavage patterns are monitored in the presence of lead(II) and other metals cations. Small molecule ligands are also studied to determine their ability to enhance or inhibit lead(II)-catalyzed cleavage of tRNA[superscript Phe]. Gel bands are quantified to determine ligand-binding equilibrium constants for the small molecule ligand-tRNA[superscript Phe] adducts. By pooling class data from the metal and small molecule ligand experiments, students draw conclusions regarding how metal-catalyzed hydrolysis is affected by various parameters such as tRNA[superscript Phe] structure, ligand charge, and inorganic chemistry of the metal ion complexes. (Contains 2 tables, 1 figure and 2 notes.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.org
Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A