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ERIC Number: EJ826717
Record Type: Journal
Publication Date: 2008-May
Pages: 2
Abstractor: As Provided
Reference Count: 8
ISSN: ISSN-0021-9584
UV Thermal Melting Curves of tRNA[superscript Phe] in the Presence of Ligands
Kirk, Sarah R.; Silverstein, Todd P.; McFarlane Holman, Karen L.
Journal of Chemical Education, v85 n5 p674-675 May 2008
This laboratory project is one component of a semester-long advanced biochemistry laboratory course that uses several complementary techniques to study tRNA[superscript Phe] conformational changes induced by ligand binding. In this article we describe a set of experiments in which the thermal unfolding of tRNA[superscript Phe] is studied with UV-vis spectrophotometry. Over the course of two laboratory periods, students examine the effects of magnesium and small molecule ligands on tRNA[superscript Phe] conformational stability. The data collected in this laboratory are fit to saturation binding curves, the ligand-binding equilibrium constants are determined, and conclusions are drawn about tRNA[superscript Phe] ligand binding modes and the impact of various types of ligands on tRNA structure. (Contains 1 table.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A