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ERIC Number: EJ826716
Record Type: Journal
Publication Date: 2008-May
Pages: 8
Abstractor: As Provided
Reference Count: 34
ISSN: ISSN-0021-9584
Probing Changes in the Conformation of tRNA[superscript Phe]: An Integrated Biochemistry Laboratory Course
Kirk, Sarah R.; Silverstein, Todd P.; McFarlane Holman, Karen L.; Taylor, Buck L. H.
Journal of Chemical Education, v85 n5 p666-673 May 2008
We have designed a new guided-inquiry laboratory for an advanced biochemistry course. This integrated laboratory focuses on the biomolecule tRNA[superscript Phe] and combines elements of bioorganic and bioinorganic chemistry with biochemistry. Throughout the semester students work together to study tRNA[superscript Phe] structure and ligand binding by (i) assaying metal-catalyzed hydrolysis using gel electrophoresis; (ii) probing thermal denaturation with UV-vis spectrophotometry; and (iii) characterizing ligand associated changes in conformation via fluorescence spectroscopy. Students begin the course with a common laboratory experience and then diverge into parallel projects, ending with a novel self-designed project. In their final report, students interpret experimental results from the entire class and draw conclusions regarding the effects of various ligands on the structure of tRNA[superscript Phe]. (Contains 1 scheme, 2 tables, 13 figures and 6 notes.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A