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ERIC Number: EJ825812
Record Type: Journal
Publication Date: 2008-Mar
Pages: 3
Abstractor: As Provided
Reference Count: 19
ISSN: ISSN-0021-9584
Radical Quenching of 1,1-Diphenyl-2-picrylhydrazyl: A Spectrometric Determination of Antioxidant Behavior
Berger, John M.; Rana, Roshniben J.; Javeed, Hira; Javeed, Iqra; Schulien, Sandi L.
Journal of Chemical Education, v85 n3 p408-410 Mar 2008
Radical chemistry is a fundamental subject of undergraduate organic chemistry. While well described in organic textbooks, laboratory manuals rarely address this topic, focusing instead on syntheses using polar reactions. Herein, we describe a colorimetric assay using the persistent radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) to measure the antioxidant activity of common antioxidants in one (3 hour) lab period. While the laboratory procedures are designed for a second-year organic laboratory environment, minor modifications to either the experiment or the supplemental material would make this suitable for more or less advanced courses. This exercise is particularly appropriate for a course geared for nutrition or food science majors. (Contains 1 figure, 1 scheme, 1 table and 2 notes.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A