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ERIC Number: EJ823329
Record Type: Journal
Publication Date: 2008
Pages: 3
Abstractor: ERIC
Reference Count: 10
ISBN: N/A
ISSN: ISSN-0002-7685
Mapping Linked Genes in "Drosophila Melanogaster" Using Data from the F2 Generation of a Dihybrid Cross
Marshall, Pamela A.
American Biology Teacher, v70 n9 p554-556 Nov-Dec 2008
"Drosophila melanogaster" is a commonly utilized organism for testing hypotheses about inheritance of traits. Students in both high school and university labs study the genetics of inheritance by analyzing offspring of appropriate "Drosophila" crosses to determine inheritance patterns, including gene linkage. However, most genetics investigations with "Drosophila" analyze offspring patterns of the F2 generation of dihybrid crosses to determine that genes are linked but do not calculate the map units between the linked genes. Calculating map units between linked genes is most straightforward when testcross data is used. However, setting up a testcross is not trivial. Constructing a testcross in "Drosophila" requires obtaining an F1 virgin female fly to mate with a homozygous recessive male fly in order to produce the subsequent generation for analysis of traits. In a teaching lab setting in which there are severe constraints on lab time, students have great difficulty in obtaining virgin F1 females to set up the testcross to generate data for mapping. This article describes how to use F2 data generated from an F1 sibmate cross to determine map distances in linked genes. (Contains 1 table.)
National Association of Biology Teachers. 12030 Sunrise Valley Drive # 110, Reston, VA 20191. Tel: 800-406-0775; Tel: 703-264-9696; Fax: 703-264-7778; e-mail: publication@nabt.org; Web site: http://www.nabt.org
Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A