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ERIC Number: EJ820785
Record Type: Journal
Publication Date: 2007-Sep
Pages: 4
Abstractor: As Provided
Reference Count: 12
ISBN: N/A
ISSN: ISSN-0021-9584
An Improved Method for Studying the Enzyme-Catalyzed Oxidation of Glucose Using Luminescent Probes
Bare, William D.; Pham, Chi V.; Cuber, Matthew; Demas, J. N.
Journal of Chemical Education, v84 n9 p1511-1514 Sep 2007
A new method is presented for measuring the rate of the oxidation of glucose in the presence of glucose oxidase. The improved method employs luminescence measurements to directly determine the concentration of oxygen in real time, thus obviating complicated reaction schemes employed in previous methods. Our method has been used to determine kinetic parameters including V[subscript max], K[subscript m], and k[subscript cat], all in good agreement with published values. The method can also be used to investigate specificity and inhibition. Information is included pertaining to the construction of a low-cost fluorescence instrument suitable for the measurements in this protocol. (Contains 3 figures.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.org
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A