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ERIC Number: EJ820784
Record Type: Journal
Publication Date: 2007-Sep
Pages: 3
Abstractor: As Provided
Reference Count: 24
ISBN: N/A
ISSN: ISSN-0021-9584
Determination of the Subunit Molecular Mass and Composition of Alcohol Dehydrogenase by SDS-PAGE
Nash, Barbara T.
Journal of Chemical Education, v84 n9 p1508-1510 Sep 2007
SDS-PAGE is a simple, rapid technique that has many uses in biochemistry and is readily adaptable to the undergraduate laboratory. It is, however, a technique prone to several types of procedural pitfalls. This article describes the use of SDS-PAGE to determine the subunit molecular mass and composition of yeast alcohol dehydrogenase employing state-of-the-art methods that produce accurate results. Students apply precise amounts of protein to the gel to determine the optimal amount required for accurate measurements. Precision Plus Protein standards are used to produce a standard curve. Students calculate the true subunit molecular mass of the protein using information from the ExPASy protein knowledgebase. They use published data from gel filtration chromatography to determine the molecular mass of the native protein and use this information and their experimental data to determine the subunit stoichiometry of the protein. (Contains 1 table and 3 figures.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.org
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A