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ERIC Number: EJ820782
Record Type: Journal
Publication Date: 2007-Sep
Pages: 3
Abstractor: As Provided
Reference Count: 25
ISSN: ISSN-0021-9584
Quantitative HPLC Analysis of Rosmarinic Acid in Extracts of "Melissa officinalis" and Spectrophotometric Measurement of Their Antioxidant Activities
Canelas, Vera; da Costa, Cristina Teixeira
Journal of Chemical Education, v84 n9 p1502-1504 Sep 2007
The students prepare tea samples using different quantities of lemon balm leaves ("Melissa officinalis") and measure the rosmarinic acid contents by an HPLC-DAD method. The antioxidant properties of the tea samples are evaluated by a spectrophotometric method using a radical-scavenging assay with DPPH. (2,2-diphenyl-1-picrylhydrazyl). Finally the students are asked to correlate the measured antioxidant properties of the different teas with their contents in rosmarinic acid. (Contains 5 figures.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A