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ERIC Number: EJ820291
Record Type: Journal
Publication Date: 2006-Jul
Pages: 4
Abstractor: As Provided
Reference Count: 15
ISSN: ISSN-0021-9584
Circular Dichroism Method for Heat Capacity Determination of Proteins
Jones, Cecil L.; Bailey, Chris; Bheemarti, Kiran Kumar
Journal of Chemical Education, v83 n7 p1067-1070 Jul 2006
Circular dichroism spectroscopy was used to measure the thermal unfolding of bovine pancreatic ribonuclease A (RNase A) with various concentrations of guanidine hydrochloride (GuHCl). A red shift in transition midpoint temperatures, T[subscript m], occurred with increasing concentration of the strong protein denaturant. van Hoff enthalpy changes, [delta]H[degree], were calculated and plotted as a function of T[subscript m] to determine the heat capacity change, [delta]C[subscript p], for denaturation. A value of 4.02 plus or minus 0.02 kJ mol[superscript -1] K[superscript -1] was calculated from d([delta]H)/d([delta]T[subscript m]). Reported values for [delta]C[subscript p] range from 4.2 to 9.6 kJ mol[superscript -1] K[superscript -1]. The shift in T[subscript m] for RNase A with increasing concentration of GuHCl suggests that the protein is undergoing substantial changes in its secondary structure.(Contains 6 figures and 1 table.)
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A