The potential impact of on-line learning in health care is significant. By providing access to educational material from an internet-connected computer anytime and anywhere, healthcare workers (HCWs), whose workload demands are often changing and somewhat unpredictable, have increased ability to self-educate. For example, the growing recognition of the importance of infection control education among HCWs in the prevention of infectious diseases has reinforced the need for accessible and timely content delivery in this particular area. Attractive as its potential may be, it is imperative that the on-line learners easily obtain access to appropriate learning programs and are satisfied with this type of learning environment in order for on-line learning to be a useful educational strategy for these adult learners. This article outlines the introductory and promotional strategies as well as the initial results and adult learner satisfaction surveys of an on-line infection control course during the first five months of the course's introduction. The objectives of this phase of the project were to: (a) evaluate the ability of the module to be accessed by healthcare workers; (b) to assess the ability of participants to learn from the on-line module; and (c) to assess the satisfaction of adult learners working in healthcare with this form of content delivery. (Contains 2 figures and 1 table.)