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ERIC Number: EJ804672
Record Type: Journal
Publication Date: 2008
Pages: 3
Abstractor: ERIC
Reference Count: 1
ISSN: ISSN-1470-8175
Problem-Solving Test: Targeted Gene Disruption
Szeberenyi, Jozsef
Biochemistry and Molecular Biology Education, v36 n4 p299-301 Jul-Aug 2008
Mutational inactivation of a specific gene is the most powerful technique to analyze the biological function of the gene. This approach has been used for a long time in viruses, bacteria, yeast, and fruit fly, but looked quite hopeless in more complex organisms. Targeted inactivation of specific genes (also known as knock-out mutation) in mice is arguably one of the most significant achievements in modern biology. The test presented in this article describes the procedure developed in the laboratory of one of the Nobel laureates of 2007, Mario Capecchi. Terms to be familiar with before one starts to solve the test: mutation, vector, plasmid, origin of replication, promoter, introns/exons, open reading frame, transfection, circular and linear DNA, DNA integration, homologous recombination, DNA replication, gene expression, heterozygote, and homozygote. (Contains 2 figures.)
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Publication Type: Guides - Classroom - Teacher; Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A