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ERIC Number: EJ804668
Record Type: Journal
Publication Date: 2008
Pages: 5
Abstractor: As Provided
Reference Count: 25
ISSN: ISSN-1470-8175
A Laboratory Demonstration Illustrating Bioseparations Using Colorful Proteins
Cohen, Theodore M.; Rohs, Amanda E.; Lefebvre, Brian G.
Biochemistry and Molecular Biology Education, v36 n4 p287-291 Jul-Aug 2008
A simple in class laboratory illustrating the principles of ion exchange chromatography as a bioseparation technique is described. A protein's isoelectric point as a driving force for ion exchange chromatography is easily demonstrated by using combinations of proteins with natural color or fluorescence, such as DsRed2, enhanced green fluorescent protein (EGFP), and flavodoxin. Simple equipment is required for this separation which can be performed on a desk top. The experiment can stimulate a range of discussion about process parameters that would create a more efficient separation of the proteins, other types of chromatography, and bioseparations in general. (Contains 4 tables and 4 figures.)
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A