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ERIC Number: EJ789309
Record Type: Journal
Publication Date: 2008
Pages: 4
Abstractor: ERIC
Reference Count: 1
ISBN: N/A
ISSN: ISSN-1470-8175
Problem-Solving Test: RNA and Protein Synthesis in Bacteriophage-Infected "E. coli" Cells
Szeberenyi, Jozsef
Biochemistry and Molecular Biology Education, v36 n2 p153-156 Mar-Apr 2008
The classic experiment presented in this problem-solving test was designed to identify the template molecules of translation by analyzing the synthesis of phage proteins in "Escherichia coli" cells infected with bacteriophage T4. The work described in this test led to one of the most seminal discoveries of early molecular biology: it dealt a lethal blow to one of the misconceptions surrounding the mechanism of protein synthesis. Terms students should be familiar with before beginning to solve the test include: Bacteriophage, "E. coli," template, translation, density labeling, radioactive labeling, ribosomes, ribosomal fraction, ribosomal subunits, cesium chloride density gradient centrifugation, sucrose density gradient centrifugation, ribosomal RNA (rRNA), and messenger RNA (mRNA). (Contains 4 figures and 1 footnote.)
John Wiley & Sons, Inc. Subscription Department, 111 River Street, Hoboken, NJ 07030-5774. Tel: 800-825-7550; Tel: 201-748-6645; Fax: 201-748-6021; e-mail: subinfo@wiley.com; Web site: https://secure.interscience.wiley.com/cgi-bin/jhome/112782101
Publication Type: Guides - Classroom - Teacher; Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A