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ERIC Number: EJ789304
Record Type: Journal
Publication Date: 2008
Pages: 4
Abstractor: Author
Reference Count: 16
ISSN: ISSN-1470-8175
An Integrated Protein Chemistry Laboratory: Chlorophyll and Chlorophyllase
Arkus, Kiani A. J.; Jez, Joseph M.
Biochemistry and Molecular Biology Education, v36 n2 p125-128 Mar-Apr 2008
Chlorophyll, the most abundant pigment in nature, is degraded during normal plant growth, when leaves change color, and at specific developmental stages. Chlorophyllase catalyzes the first chemical reaction in this process, that is, the hydrolysis of chlorophyll into chlorophyllide. Here, we describe a series of laboratory sessions designed to illustrate a sequence of experiments used as part of the scientific research process and to convey key biochemical concepts. The format guides students through the process of biochemical protein analysis, starting from a recombinant protein expression vector and working through a kinetic analysis of the purified protein. Over the course of these experiments, students learn protocols in basic protein chemistry that allow them to design and conduct a related experiment of their own interest. The described set of laboratories can be tailored to fit either a 4- or an 8-week series of experiments for use in either introductory or advanced biochemistry laboratory courses, respectively. (Contains 4 figures and 1 footnote.)
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A