ERIC Number: EJ780210
Record Type: Journal
Publication Date: 2007
Reference Count: 20
Conjugation in "Escherichia coli"
Phornphisutthimas, Somkiat; Thamchaipenet, Arinthip; Panijpan, Bhinyo
Biochemistry and Molecular Biology Education, v35 n6 p440-445 Nov-Dec 2007
Bacterial conjugation is a genetic transfer that involves cell-to-cell between donor and recipient cells. With the current method used to teach students in genetic courses at the undergraduate level, the transconjugants are identified using bacterial physiology and/or antibiotic resistance. Using physiology, however, is difficult for both first-year undergraduates and special science students at the high school levels, who do not have the basic knowledge. We have developed a laboratory exercise that comprises a simple and rapid technique for transferring bacterial DNA by conjugation and examining the transconjuants using only antibiotic resistance on agar. The identity of the right transconjuants is confirmed by agarose gel electrophoresis. This exercise is designed to help students understand how horizontal gene transfer occurs in bacteria by conjugation using "Escherichia coli" as a hands-on learning model. Students should be able to draw concept maps of three DNA transfer methods on their own after carrying out the experiment and getting some additional information. (Contains 3 tables, 2 figures, 1 footnote, and 2 appendices: (1) "Plasmid Nomenclature"; and (2) "Transformation Method.") [This work was partially supported by the Teacher Professional Development Project (TPDP) jointly administered by the Ministry of Science and Technology and the Institute for the Promotion of Teaching Science and Technology (IPST).]
Descriptors: Concept Mapping, Science Activities, Physiology, Genetics, Teaching Methods, Molecular Biology, Biochemistry, Science Instruction, Laboratory Experiments, Undergraduate Students, College Science, Learning Activities, Foreign Countries
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Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Sponsor: Ministry of Education (Thailand).
Authoring Institution: N/A
Identifiers - Location: Thailand