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ERIC Number: EJ769514
Record Type: Journal
Publication Date: 2007-Jul
Pages: 7
Abstractor: Author
Reference Count: N/A
ISBN: N/A
ISSN: ISSN-1470-8175
From Egg to Crystal: A Practical on Purification, Characterization, and Crystallization of Lysozyme for Bachelor Students
Olieric, Vincent; Schreiber, Angelique; Lorber, Bernard; Putz, Joern
Biochemistry and Molecular Biology Education, v35 n4 p280-286 Jul 2007
A practical hands-on course encompassing enzyme purification, biochemical characterization, and crystallization that completed the course work of 350 second-year bachelor students enrolled in molecular biology/biochemistry was given at the Universite Louis Pasteur of Strasbourg (France). The experimental part of the practical dealt entirely with the model protein lysozyme isolated from hen egg-white. It was designed as a research project to give students the possibility to practice biochemical methods such as chromatography, electrophoresis, and spectrophotometry. It also included enzyme activity assay and protein crystal growth that are usually taught in master-level courses. The organization of the practical work and the related experimental procedures are described and discussed.
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Publication Type: Journal Articles; Reports - Evaluative
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: France