This research note provides a rationale for the use of gender-specific subscales, rather than a single global measurement device, to assess respondents' differential attitudes toward lesbians and gay men. It then evaluates the reliability and validity of equivalent forms of the Attitudes toward Lesbians and Gay Men scale (ATLG) (Herek, 1994) when administered to professional social workers. Following the psychometric testing of these instruments, the responses of 288 heterosexual social workers to each of the items of the Attitudes Toward Gay Men (ATG) (Herek, 1994) and the attitudes toward lesbians (ATL) (Herek, 1994) subscales were compared.