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ERIC Number: EJ401417
Record Type: CIJE
Publication Date: 1989
Pages: N/A
Abstractor: N/A
Reference Count: 0
A Versatile and Inexpensive Enzyme Purification Experiment for Undergraduate Biochemistry Labs.
Farrell, Shawn O.; Choo, Darryl
Journal of Chemical Education, v66 n8 p692-93 Aug 1989
Develops an experiment that could be done in two- to three-hour blocks and does not rely on cold room procedures for most of the purification. Describes the materials, methods, and results of the purification of bovine heart lactate dehydrogenase using ammonium sulfate fractionation, dialysis, and separation using affinity chromatography and elution. (YP)
Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: Teachers; Practitioners
Language: English
Sponsor: N/A
Authoring Institution: N/A