The purpose of this study was to examine the effects of tonic drone accompaniments on the intonation of collegiate wind instrumentalists. Participants (N = 68) played an excerpt of the melody "Long, Long Ago" in three conditions: a mono drone (tonic note only), dyad drone (tonic plus fifth), and a control condition (no drone). Results indicated no significant effects on intonation performance due to drone condition. However, participants' ratings of their own intonation accuracy differed significantly based on drone condition. The majority of the performances of the melody aligned more closely with equal temperament (n = 159), and fewer aligned more closely with just intonation (n = 45). Most participants believed the dyad drone (59.74%) resulted in their best intonation accuracy, followed by the mono drone (28.57%) and the control condition (11.68%). In response to open-ended questions, participants cited reasons why they preferred particular drone conditions, with the most common themes being "easier to hear and match," "multiple reference pitches," and "focused/directed listening." Given that participants expressed preferences regarding drone use in the absence of performance differences, music educators may consider the role of comfort and familiarity with these instructional tools.