Misconceptions are a major problem in learning biology and their emergence may potentially increase during online lectures. The purpose of this study was to identify student misconceptions in Department of Biology Education, Universitas Muhammadiyah Malang during online biochemistry lectures. This quantitative study involved 39 respondents. Two-tier questions were used as data collection instruments and descriptive statistics were chosen as data analysis techniques. As a result, the average percentage of students who mastered the concepts delivered correctly was 40.4%. Meanwhile, the average percentage of students who experienced misconceptions was 53.0%. The high level of misconceptions found in this study needs to be followed up by improving the quality of online lectures during the pandemic.