This study aimed to investigate and compare the perception of TPACK competence of graduate students studying at the institutes of Natural Sciences, Social Sciences and Educational Sciences based on the variables of gender, graduate program level and field. The participants of the research consist of 186 graduate students studying at the institutes of Karatay, Necmettin Erbakan and Selçuk University. TPACK Competence Scale was used to collect research data. The results of the research showed that graduate students' technological knowledge and TPACK competence were moderate. It was also found that male graduate students' technological knowledge and technological content knowledge were high, whereas female participants had a high-level perception of pedagogical knowledge. In addition, perceptions of TPACK competence varied based on the field and level of graduate education.