Culture provides the main background of knowledge increase. As an important sub culture, academic culture has a large impact on the development of knowledge creation. This research studies academic culture and knowledge management in higher education system. For the purpose of study, a sample including academic scholars and experts (academic staff members) was chosen using objective sampling and interviewed. Interviews continued until implicit effective cultural aspects and factors on the knowledge management of academic staff members were identified and described. Qualitative stage of current study led to the identification of effective cultural factors on knowledge management. Additionally, the constructs of knowledge culture in higher education system was identified considering research theoretical bases and findings.