Context: Telemedicine is the practice of providing diagnostic consultations and therapeutic interventions to patients at a distance using some form of technology. Typically, health care students do not have the opportunity to practice telemedicine. Objective: To investigate athletic training students' ability to transfer telemedicine skills confidently and accurately in a standardized patient (SP) encounter. Design: Single cohort. Setting: Simulation center. Patients or Other Participants: Fifty-five second-year athletic training students (age = 25 ± 3 years) from 6 professional master's athletic training programs volunteered for the study after a 1-week online learning experience about telemedicine. Intervention(s): We scheduled individual SP encounters that were completed at a distance using telepresence robots. Main Outcome Measure(s): Pre- and post-SP encounter validated confidence assessment and a 50-item content checklist (yes or no) scored by one evaluator. Results: During the SP encounter, 87.3% of participants correctly diagnosed the SP actor with a lateral ankle sprain. We identified a significant improvement in confidence (P [less than or equal to] 0.001) for using telemedicine technology. On the content checklist, participants scored poorly in the constructs of data gathering (mean = 7.44 ± 2.36 of 15, 49.58% ± 15.75%) and telemedicine (mean = 6.02 ± 2.74 of 14, 42.99% ± 19.56%), but scored well in the constructs of communication/ interpersonal skills (mean = 12.05 ± 2.00 of 15, 80.36% ± 13.36%) and patient education (mean = 4.64 ± 1.06 of 6, 77.27% ± 17.67%). The mean sum score of all constructs on the SP encounter was moderate (30.15 ± 5.79 of 50, 60.29% ± 11.59%). Conclusions: Exposure to telemedicine via an SP encounter improved confidence in performing the tech-based evaluation. Athletic training students performed well in demonstrating communication/interpersonal skills and patient education, yet struggled in their data gathering and telemedicine skills. Overall, participants accurately diagnosed a musculoskeletal condition using telemedicine.