This study aimed to test a hypothetical path model estimating the influence of Type D personality on health-promoting behaviors (HPB), subjective health status, and quality of life (QoL) in university students, and to verify the mediating effects of HPB and subjective health status between Type D personality and QoL. A structural equation model involving 380 university students was used. Data were collected between November and December 2018 via structured questionnaires and analyzed using SPSS 25.0 and Amos 23.0. The model's fit indices were adequate. Type D personality had a direct effect on QoL and an indirect effect on QoL via HPB and subjective health status. Type D personality, HPB, and subjective health status explained 67.6% of the total variance in QoL. Intervention programs that consider Type D personality, HPB, and subjective health status may be useful to improve university students' QoL.