While previous studies have examined lesbian, gay, bisexual, transgender, and queer (LGBTQ) information needs, none have addressed librarian confidence in addressing LGBTQ-themed information needs or the factors affecting this confidence. The authors used a mixed-methods survey to assess the knowledge and perspectives of academic librarians in responding to information inquiries related to sexual orientation and gender identity. Based on an exploratory factor analysis, three variables were identified: duty of care/vulnerability of inquirer, public visibility of work conducted, and personal biases and prejudices. These factors can reduce or otherwise influence the ability to meet LGBTQ information needs.