Collaborative innovation, with universities as the main body, is an important foundation for deepening the cooperation between industry, universities, and research institutes. Taking the collaborative innovation of five universities in Japan Shikoku area as an example, this paper summarizes the content of collaborative innovation in colleges and universities. The Shikoku SICO, which is established by government, is set to integrate the resource of university, enterprises, industry, and government, expand and broaden the knowledge chain, and promote knowledge flow and value realization through knowledge gathering and diffusion, knowledge dissemination and sharing, and knowledge transfer and application. Based on SICO's collaborative innovation mode of the Japan Shikoku area, five universities have established a virtuous circle of knowledge, capital, and talents, forming a collaborative innovation ecosystem characterized by symbiosis. From an ecology perspective, this paper establishes a regional collaborative innovation symbiotic system, which is characterized by knowledge, with the components of producer, consumer, decomposer, and catalyzer. Finally, from the perspective of constructing symbiosis system, this paper puts forward the experience of colleges and universities in Japan in terms of knowledge dissemination, knowledge transfer, and knowledge gathering.