The study investigated the attitudes of Biology teachers and students toward conservation of natural resources in Ekiti State. The participants for this study were 50 Biology teachers and 100 Biology students. The selection was based on purposive sampling technique which involved 10 secondary schools. Data collected from teachers' and students on conservation of Natural Resources Questionnaire were analyzed using frequency counts, percentage, mean, standard deviation and t-test. The findings indicated that both teachers and students had a good knowledge of natural resources conservation concepts. The attitudes of both teachers and students point more to positive than negative. On a general note, the teachers had higher mean scores than the students indicating that teachers tended to have more favourable attitudes than the students toward natural resources conservation. It is recommended therefore, that the Federal and State environmental protection agencies should continue to make schools their principal focus in their bids to create, promote and sustain public awareness of the importance of natural resources conservation to mankind.