e-Bug, an international educational resource for educators to teach children about hygiene and antibiotics, developed free e-Learning for educators to increase knowledge about e-Bug and develop skills to optimise use. Educators completed an online evaluation survey after completing the e-Learning. Quantitative data were analysed using Microsoft Excel. Two qualitative focus groups explored educator views; data were thematically analysed. One hundred participants participated. Overall impression of the e-Learning was very good/good (88%). Eighty per cent stated that their perceived knowledge of e-Bug improved after completing the training. Four main themes emerged from qualitative data: attributes of the e-Learning, perceived educator self-development, promotion and accreditation of educator training, and the delivery of educator training. Educators suggested modifications. Authors conclude that e-Learning is a suitable tool to aid educator learning; educators view the e-Bug e-Learning as a valuable continuous professional development resource to improve educator knowledge, confidence and skills to teach about hygiene topics.