Positive attitude towards statistics is a desirable aspect in developing statistical thinking and understanding statistical concept. The attitude towards statistics instrument in previous study on rural school students lacked evidence in the validity analysis. Therefore, there is a need to provide a valid instrument that can assess rural secondary school students' attitude toward statistics. For this purpose, this study was carried out to investigate the validity and reliability analysis of the Survey Attitude towards Statistics (SATS-36) instrument from a sample of rural secondary school students. The SATS-36 is a 7-point Likert type scale questionnaire, used to measure students' attitude towards statistics. The SATS-36 instrument was distributed to 217 Tenth grade science stream students from a rural district in Sabah, Malaysia, which comprised of 66 (30.4%) boys and 151 (69.6%) girls. A second order Confirmatory Factor Analysis (CFA) was carried out to analyze the validity of the SATS-36 instrument as well as to confirm the 6-factor model of SATS-36. The reliability of SATS-36 instrument was assessed through the Cronbach's Alpha value. Overall, the analysis showed that the SATS-36 instrument shows favorable indices for both its validity and reliability. Thus, it can be concluded that the SATS-36 was a valid and reliable instrument for measuring students' attitude towards statistics among rural science stream secondary school students.