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ERIC Number: EJ1234196
Record Type: Journal
Publication Date: 2019
Pages: 4
Abstractor: As Provided
ISSN: ISSN-1470-8175
An Idea to Explore: Genotyping Bull Sperm to Introduce Basic Molecular Biology Techniques in an Animal Science Course
De Groef, Bert; Grommen, Sylvia V. H.
Biochemistry and Molecular Biology Education, v47 n6 p708-711 Nov-Dec 2019
The laboratory exercise described here aims to provide a relevant context for learning basic DNA techniques in an introductory animal science course at tertiary level. In two 4-hr laboratory sessions, students assess the suitability of bulls for inclusion in a gene-assisted selection program for A2 [beta]-casein by genotyping commercial bull sperm. Sperm cells are lysed to extract the genomic DNA, and PCR with primers for the ß-casein gene is performed. Using the principle of amplification-created restriction sites, restriction digestion with "Taq"I can be used to distinguish between the "A1" allele and the "A2" allele of the gene. Cut PCR amplicons are separated by gel electrophoresis to evaluate the genotype of each bull. Students then write a diagnostic report with accompanying letter to their fictional client, explaining the DNA test, and interpreting the results.
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A